Smad4 sc-7966
WebbSMAD4 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue … WebbSuitable control antibody: Smad4 Antibody (B-8): sc-7966 is recommended as control antibody for monitoring of Smad4 expression knockdown by Western blotting or immunofluorescence Get details about si/shRNA Gene Silencers Gene silencers are intended for noncommercial and research use only. Click here for details. See product …
Smad4 sc-7966
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WebbSmad proteins are phosphorylated and activated by transmembrane serine-threonine receptor kinases in response to TGF-beta signaling. ... (GTX112980) diluted at 1:1000 … Webb18 nov. 2016 · Although Smad4 clearly has crucial roles in skeletal muscle and tissue-specific stem and progenitor cell biology, to date no studies have explicitly examined whether or not there is a cell-autonomous requirement for Smad4 in SCs and derived MPs during skeletal muscle regeneration.
Webb5 mars 2003 · Smad4 is a tumor suppressor gene that is commonly lost or mutated in colorectal and pancreatic cancers. The activated transforming growth factor- β (TGF- β) … Webbcatalog: sc-7966 total number of products: 8. ... Smad4 (SPM448) Antibody Santa Cruz Biotechnology catalog: sc-56479 total number of products: 8. mouse monoclonal (SPM448) reactivity: human, mouse, rat application: western blot, immunocytochemistry, immunoprecipitation citations: 2.
Webband a mouse anti‑Smad4 antibody (1:100; sc‑7966; Santa Cruz Biotechnology, Santa Cruz, CA, USA) were used as a primary antibody. Following an overnight incubation with the … WebbTo establish the Smad4-expressing BxPC3 cells, we transfected 2μg pcDNA3 Flag-Smad4 vector to BxPC3 cells and selected as described previously. (2)The control cell line B3EV was established as described previously. (8) RNA interference. RNA interference against Smad4 has been described previously.
Webb25 maj 2024 · SMAD4, a key regulator of transforming growth factor-β (TGF-β) signaling, plays a major role in cell growth, migration, and apoptosis. In particular, TGF-β/SMAD …
WebbCheck out videos that might be relevant for performing Western blotting Smad4 using Smad4 Antibody (B-8): sc-7966 from Santa Cruz Biotechnology. Please note that these … small things movieWebbWe assayed Smad4 by performing immunohisto- chemistry using a mouse monoclonal anti-Smad4 antibody (sc-7966; dilution, 1:50; Santa Cruz Biotechnology, CA). The specificity of this anti-Smad4 antibody is described elsewhere (8). small things nthikeng mohlele pdfWebbDownload scientific diagram Smad3 and Smad4 cooverexpression represses endogenous c- myc expression. (A) BALB/MK cells either were treated with 400 nM TGF-  or vehicle … small things netflixWebb8 mars 2024 · Mouse anti-Smad4 (sc-7966) and rabbit anti-Smad1/5/8 (sc-6031) were purchased from Santa Cruz Biotechnology (Dallas, TX). 2.3.4 Alkaline phosphatase … highway that goes through the most statesWebbSAN TA C RUZ BI OTEC HNOL OG Y, INC . Smad4 (N-16): sc-1908 SantaCruzBiotechnology,Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com BACKGROUND Smad proteins, the mammalian homologs of the Drosophila mothers against dpp (Mad) have been … highway that goes underwaterWebb17 mars 2009 · Abstract. Transforming growth factor-β (TGF-β) functions as a tumor suppressor of the prostate through mechanisms that remain unresolved. Although TGF … highway that plays america the beautifulWebb24 aug. 2013 · All incubations with anti-SMAD4 antibody (B-8, sc-7966, Santa Cruz, USA) at 1:200 dilutions were carried out overnight at 4 °C. Then, the specimens were briefly washed in PBS (3×5 min) and incubated at room temperature with anti-rabbit antibody and then streptavidin–biotin protein peroxidase (Maixin Fuzhou, China). small things nthikeng mohlele notes