Phis2图谱
Webb1 juni 2024 · Clone the products of the PCR reactions carried out in step 3 into the pHIS2 T-vector obtained in step 5. The T-A ligation system includes 0.2 μg of T-vector of pHIS2, 0.5 μL of PCR products (Y1 + Y3 and Y2 + Y3), 1 μL of 10× ligation buffer, 3 U of T4 ligase, and PEG6000 at final concentration of 10% (w/v). Webb19 nov. 2012 · 是选取atg上游2000的片段,然后整个连到phis2载体上吗? 我之前做了直接取ATG上游1.5k构建到phis2上,感觉不合适。 最好还是找到自己转录因子的结合motif然后取motif和其上下游几十bp的片段,然后串联3个片段构建载体比较合适。
Phis2图谱
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WebbPlasmid pHIS2-hsSTAM1 (1-143) from Dr. James Hurley's lab contains the insert phis2- STAM1(1-143) and is published in Structure. 2009 Mar 11. 17(3):406-16. This plasmid is … http://www.youbio.cn/product/vt1375
WebbWith the development of pGADT7-Rec2 (and pHIS2, a low-copy reporter vector), integration is no longer necessary because each plasmid, with its CEN and ARS elements, now behaves like a minichromosome, both mitotically and meiotically. Use. pGADT7-Rec2 is engineered for constructing GAL4 AD/cDNA libraries by homologous recombination in … WebbPartial Sequences from Depositor (1) Sequence provided by depositing laboratory may be theoretical/predicted or based on Sanger/NGS sequencing results. Discrepancies between sequencing results obtained by Addgene and the original sequence provided by the depositor may be present. Analyze Sequence. GenBank.
Webb21 mars 2024 · 为了揭示可以操纵香豆素合成的转录因子,我们基于40个苹果枝条样本的转录组图谱,使用wgcna构建了与香豆素合成相关的基因的共表达网络(图7a)。 基于平均层次聚类和动态树木修剪确定了七个模块,并且ME绿松石模块与这些香豆素相关性状的相关性最高(图7B)。 WebbpHis2载体价格1000元,pHis2载体质粒图谱(Vector map),载体序列(Sequence)见下文,载体质粒抗性为Kanamycin,质粒大小为7207bp。pHis2是酵母单杂交载体的pHis2。
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http://www.biofeng.com/zaiti/jiaomu/pHis2.html iowaassessorlucasWebbpHIS2质粒. pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterizeDNA-binding proteins. The vector was specifically designed for use with the BD Matchmaker™ One-Hybrid Library Construction & … onyx ho302Webbxho1和sac1的酶切位点是什么? 它识别的特定基因是体内3广a泛存在的siRNA和miRNA前体。Dicer酶的PAZ结构域特异性地识别 0’端有 5个j碱基突出的 dsRNA,之w后由RNase III结构域剪切8生成siRNA或microRNA。 2011-10-27 8:58:59 onyx herstellerhttp://www.honorgene.com/uploads/files/202411/6181dfe1813d2.pdf onyx ho368Webb10 nov. 2015 · 植物表达载体 pcambia1301(EHA105). 可提供近万种质粒载体、菌株、基因和细胞株,并可提供实验技术外包服务,如基因克隆、质粒构建、蛋白原核、真核表达及纯,pQE 载体,原核表达载体,克隆载体 ,毕赤酵母表达载体,毕赤酵母宿主菌,酿酒酵母表达载体 ,酿酒 ... iowaassessormason cityWebb20 juni 2024 · pHis2 酵母单杂交载体 -- -- 多克隆位点,限制性内切酶 7207bp -- -- -- 卡纳霉素 -- 含HIS3报告基因 -- -- -- -- 载体质粒图谱和多克隆位点信息 载体简介 载体序列 LOCUS … onyx ho124http://www.biofeng.com/zaiti/qita/pHISi.html iowaassessor johnson co