Isopropyl β-D-1-thiogalactopyranoside (IPTG) is used along with X-gal for blue-white screening. IPTG is a non-metabolizable analog of galactose that induces the expression of lacZ gene. It should be noted that IPTG is not a substrate for β-galactosidase but only an inducer. See more Blue-white screening is a rapid and efficient technique for the identification of recombinant bacteria. It relies on the activity of β-galactosidase, an enzyme occurring … See more The presence of lactose in the surrounding environment triggers the lacZ operon in E. coli. The operon activity results in the production of β-galactoisdase enzyme … See more For screening the clones containing recombinant DNA, a chromogenic substrate known as X-gal is added to the agar plate. If β-galactosidase is produced, X-gal is … See more WebChromoMax IPTG/X-Gal Catalog # 13988100 13988101 13988102 BP42001 BP420010 BP420050 SDS ChromoMax™ IPTG/X-Gal Catalog # 10133443 10225163 10658593 ACRBP4200 BP4200 BP42001 BP420010 BP420050 SDS 5-Bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-GAL), Ultrapure, Thermo Scientific Catalog # ALFAAJ10077 SDS …
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WebIPTG, X-gal, speciinomycin Based on your answers to the previous questions, fill in Table with your predictions of whether there will be bacterial growth on each plate: Okay, So 63 … WebTransform the reaction product to XL1-Blue and streak the transformant on Ampicillin/X-gal/IPTG LB plate. Culture O/N at 37°C. Screen for correctly assembled clones by colony PCR. You can use TALE-F/TALE-R primers both for ptCMV-153/47-VR and ptCMV-136/63-VR vectors. The detailed reaction condition is described in Appendix. daughtry concerts in ct 2021
Following transformation, each sample will be spread
WebStudent Guide Table 3 lists the four experimental samples (A, B, C, and Dy that you will be working with as well the conditions under which they will be grown. During the activity, … WebApr 4, 2024 · (D) The E. coli K-12 BW25113 WT and ∆ lon strains harboring the plasmid pCA24N-NHis-yefM were induced with 0.5 mM IPTG for 30 min, and 1% spectinomycin (100 µg/mL) was added to the strains to activate a stress response. We collected equivalent quantities of cells at 0 min, 30 min, 60 min and 120 min and then ran Tricine-SDS-PAGE … WebMar 16, 2010 · Pspac = IPTG inducible promoter, PdivIVA = constitutive B. subtilis divIVA promoter used to drive expression of lacZ, AmpR = ampicillin resistance gene, SpcR = spectinomycin resistance gene. Plasmid pLK has been made available through the Bacillus Genetic Stock Centre, with accession number ECE219. daughtry concert setlist