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Fastpfu polymerase

WebTransStart® FastPfu DNA Polymerase has passed the following quality control assays: functional absence of double- and single-strand endonuclease activity, >99% homogeneous measured by SDS-PAGE. Each batch of TransStart® FastPfu DNA Polymerase has been assayed for amplification efficiency to amplify p53 gene from 10 ng of human genomic DNA. WebFeb 8, 2016 · TransStart FastPfu Fly DNA Polymerase is a hotstart, ultra-high fidelity and high-processivity DNA Polymerase. It consists in a modified DNA Polymerase, increasing its affinity for dsDNA and …

福建省龙岩市珠蛋白基因新位点突变的测序检测_参考网

WebJul 6, 2024 · 包括:HiPure Tissue DNA Mini Kit(美基生物),琼脂糖(西班牙Biowest),EB (上海阿拉丁生化科技有限公司),2×Power Taq PCR MasterMix(北京百泰克生物技术有限公司),TransStart FastPfu Fly DNA Polymerase(北京全式金生物技术有限公司),根据人类11号染色体的序列(NC ... WebJul 15, 2024 · Planktonic eukaryotes are important components of aquatic ecosystems, and analyses of the whole eukaryotic planktonic community composition and function have far-reaching significance for water resource management. We aimed to understand the spatiotemporal variation and drivers of eukaryotic plankton distribution in the Middle … danny phantom wcofun https://thebodyfitproject.com

TransStart® FastPfu Fly DNA Polymerase - bosterbio.com

Web【求助】全式金(transgen)的Fast PFU酶里面的Stimulant大概是个什么东西呢,对后续反应有什么影响?. 买的全式金(transgen)的Fast PFU酶,这个酶里面有一个Stimulant母液是5×的,终浓度推荐0.5×到2.5×之间,这个东西大概是什么呢?对后面的反应有没有影响 … WebJul 22, 2024 · PCR amplification was performed using the TransStart ® FastPfu Polymerase system (Transgen Biotech, Beijing, China), including 4 μL 5 × FastPfu Buffer, 2 μL dNTPs (2.5 mM), 0.8 μL each primer (5 μM), 0.4 μL FastPfu Polymerase, and 10 ng template DNA. The reaction mixture was initially denatured at 95°C for 2 min, followed by … WebPfu DNA Polymerase is a high-fidelity, thermostable enzyme of approximately 90kDa isolated from Pyrococcus furiosus. The enzyme replicates DNA at 75°C, catalyzing the polymerization of nucleotides in … danny phantom wolf girl

TransStart® FastPfu Fly DNA Polymerase ABIN5519533

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Fastpfu polymerase

TransStart® FastPfu DNA Polymerase

WebBrand : TransGen Biotech. Manual. Product Details. TransStart® FastPfu PCR SuperMix is a ready-to-use mixture of TransStart® FastPfu DNA polymerase, dNTPs, and optimized … WebTransStart® FastPfu DNA Polymerase 是用于快速PCR的热启动高保真DNA聚合酶,TransStart® FastPfu DNA Polymerase扩增效率高、扩增速度快(4 kb/min,是普通Pfu …

Fastpfu polymerase

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WebAug 23, 2024 · The PCR mixtures contain 5 × TransStart FastPfu buffer 4 μL, 2.5 mM dNTPs 2 μL , forward primer (5 μM) 0.8 μL, reverse primer (5 μM) 0.8 μL, TransStart FastPfu DNA Polymerase 0.4 μL, template... WebFastPfu FLY (Ultra-HiFi) DNA Polymerase Setting Higher Standards of High-Fidelity DNA Polymerase Performance PCR with FLY Today ! ; Features of FastPfu FLY Highly Specific FastPfu FLY Features Un …

WebPhusion ® High-Fidelity DNA Polymerase For high speed and high performance PCR. Manufactured and quality-controlled at New England Biolabs, Thermo Scientific ® Phusion High-Fidelity DNA Polymerase offers both high fidelity and robust performance. 50X higher fidelity than Taq Robust reactions - maximal success with minimal optimization WebFeb 17, 2024 · The amplification system was 20ul, 4ul 5*FastPfu buffer, 2ul 2.5 mM dNTPs, 0.8ul primer (5uM), 0.4ul FastPfu polymerase; 10 ng DNA template. Purified amplicons were pooled in equimolar amounts and paired-end sequenced on an Illumina MiSeq PE300 platform/NovaSeq PE250 platform (Illumina, San Diego, USA) according to the standard …

WebCharacteristics. - TransStart® FastPfu Fly DNA Polymerase offers 108-fold fidelity as compared to EasyTaq® DNA Polymerase. - PCR products can be directly cloned into … WebTransStart® FastPfu Fly DNA Polymerase is a hot start, high fidelity and high processivity DNA Polymerase. TransStart® FastPfu Fly DNA Polymerase.

WebApr 10, 2024 · PCR was performed in triplicate in a 20 μL mixture containing 4 μL of 5 × FastPfu Buffer, 2 μL of 2.5 mM dNTPs, 0.8 μL of each primer (5 μM), 0.4 μL of FastPfu Polymerase, and 10 ng of template DNA.

WebNov 13, 2024 · The V3-V4 regions of the 16S ribosomal RNA gene were amplified by PCR. The reaction mixture contained 4 μL of 5 × FastPfu Buffer, 2 μL of 2.5 mM … danny phegan the voiceWebPfu Polymerase is a thermostable DNA polymerase isolated from the archaeal thermophile Pyrococcus furiosus. The 90-kDa Pfu polymerase enzyme features a 3’-5’ exonuclease proofreading ability, allowing for higher fidelity than Taq. The error rate of Pfu has been reported to be at least 10-fold lower than that of Taq. danny phegan on the voiceWebThe PCR mixtures contain 5 × TransStart FastPfu buffer 4 μL, 2.5 mM dNTPs 2 μL , forward primer (5 μM) 0.8 μL, reverse primer (5 μM) 0.8 μL, TransStart FastPfu DNA … birthday line up icebreaker