WebEasySep™ Human Positive Selection Kits Typical Sample Source: PBMCs, Leukopaks * Protocol Time: As Little As 15 Minutes Selection Method: Positive Selection Features/Advantages: High purity (up to … WebTYPICAL EASYSEP CD45 DEPLETION PROFILE: Start: 99.3% CD45+ Cells and Depleted: 1.5% CD45+ Cells 0.7% CAMA Cells (Seeded into 4.0 Log Depletion of CD45+ Cells Peripheral Blood Mononuclear Cells) Ber-EP4 is an antibody against an epithelial cell surface antigen. COMPONENT DESCRIPTIONS: EasySep® Human CD45 Depletion …
Modeling of clinical phenotypes in systemic lupus erythematosus …
WebNov 5, 2024 · A low frequency (<1%) of immune cells in the single cell suspension from a kidney biopsy core necessitated immune enrichment in 10 samples (5 males, 5 females) using magnetic EasySep Human CD45 depletion kit II as per the manufacturer’s modified instructions for positive selection of CD45-expressing cells. WebMar 12, 2024 · Deplete CD45 + cells with CD45 - negative cell isolation kit by following manufacture’s protocols. Use phenol red-free DMEM during isolation and incubate the cells at 4 ° C. Incubate the CD45 - cells with fluorescence-conjugated antibodies including AF488-CD45, PE-podoplanin, APC-CD31 and LIVE/DEAD-Near IR for 30 min at 4 o C. for rent in stuart fl
Human CD45 Depletion Kit - STEMCELL
WebEasySep™ Human Whole Blood CD45 Depletion Kit Sample Source: Whole Blood Capacity: 100 mL Recommended Applications: Enrichment of CTCs by depleting CD45+ cells. The protocol for this kit includes a red … Web3 rows · Deplete CD45+ cells from fresh or previously frozen human peripheral blood mononuclear cells ... WebApr 7, 2024 · Efficiency of CD45 + cell and red blood cell depletion was confirmed by flow cytometry at the end of the isolation protocol. (Additional file 1: Fig S1). Isolated platelets were lyzed in 500 μL QIAzol ™ (Qiagen) and stored at − 80 °C until use. digital banks for small business