Biotin repressor stress
WebAug 1, 2008 · The biotin repressor is an allosterically regulated, site-specific DNA-binding protein. Binding of the small ligand bio-5′-AMP activates repressor dimerization, which is a prerequisite to DNA binding.Multiple disorder-to-order transitions, some of which are known to be important for the functional allosteric response, occur in the vicinity of the ligand … WebNov 15, 2013 · Many biological switches including the stress response [1] and biofilm formation [2] in bacteria and inflammation [3] and development [4] in higher organisms are controlled at the level of protein:protein interactions. In each of these systems, the switch involves swapping of one protein interaction partner for another.
Biotin repressor stress
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WebEnzymatic Biotinylation: BirA. Ligation of biotin to a protein is a specific post-translational … WebNov 9, 2002 · The Escherichia coli biotin repressor, an allosteric transcriptional regulator, is activated for binding to the biotin operator by the small molecule biotinyl-5‘-AMP. Results of combined thermodynamic, kinetic, and structural studies of the protein have revealed that corepressor binding results in disorder to order transitions in the protein monomer that …
WebFeb 13, 1996 · The Escherichia coli repressor of biotin biosynthesis (BirA) is an allosteric site-specific DNA binding protein. The protein is composed of three structural domains. Contact with the biotin operator (bioO) in the transcriptional repression complex is made by the N-terminal domain which contains a helix−turn−helix structural module. The … WebNov 14, 2024 · In this study, we establish OmpR as a global regulator of acid and osmotic stress in S. Typhimurium and E. coli. OmpR represses distinct sets of genes based on the intracellular pH threshold...
WebNov 14, 2024 · Acid stress reduces the internal pH below this threshold, increasing OmpR dimerization and DNA binding. ... in the ompR null mutant, suggesting that E. coli OmpR also functions as a repressor at ... WebThe identity of the biotin repressor and the biotin-apo- BCCP ligase of E. coli was strongly indicated by the very detailed genetic studies of Barker and Campbell (3, 4) who showed that lesions in the same genetic entity, the birA gene, gave rise to both biotin-apoBCCP ligase deficiency and to depression of the biotin operon.
Web2 days ago · We found that the expression of genes related to a lytic phage lifestyle in the phage-plasmid were highly up-regulated after the observation of mutations in the repressor sequence, such as the...
WebNov 12, 2024 · OsClo5 functions as a transcriptional co-repressor by interacting with OsDi19-5 to negatively affect salt stress tolerance in rice seedlings. Pei Jing, ... The MBP-OsDi19-5 was incubated with the biotin-labeled wild-type Di19-binding sequence (DIBS) probe or mutated DIBS probe, and the non-labeled DIBS probe was used as a competitor ... how are we all relatedWebMar 24, 2006 · BirA is a bifunctional enzyme that adenylates biotin and then uses the … how many minutes is 3 hours and 32 minutesWebOct 1, 1992 · The three-dimensional structure of BirA, the repressor of the Escherichia … how many minutes is 3 hours and 30 minsWebJan 31, 2003 · The biotin repressor is an allosterically regulated, site-specific DNA-binding protein. Binding of the small ligand bio-5′-AMP activates repressor dimerization, which is a prerequisite to DNA binding. Multiple disorder-to-order transitions, some of which are known to be important for the functional allosteric response, occur in the vicinity ... how many minutes is 3 minutesWebBiotin is a member of the vitamin B-complex family. Biotin deficiency has been … how are weather and climate the sameWebMay 15, 1998 · The Escherichia coli repressor of biotin biosynthesis, BirA, binds to a 40 bp operator site (bioO) to repress transcriptional initiation at the two divergent promoters of the biotin biosynthetic operon (Figure 1A; Otsuka & Abelson, 1978). how many minutes is 4000 secondsWebMar 5, 2024 · BirA is a transcriptional repressor of the biotin biosynthesis operon ( bioO, shaded gray) in both bacteria. BirA is proposed to also regulate expression of the biotin transport protein BioY and the yhfS - yhfT operon in S. aureus. b Sequences of the promoters for bioO in E. coli and bioO, bioY, and yhfS-yhfT in S. aureus are shown. how are weapons made